Barley yellow mosaic virus (BaYMV) cause severe damage in barley cropping field especially yield reduction about 40% to 100% depend on climatic conditions and varieties. The correlations between disease incidence (DI) and changes of climatic conditions were analyzed. The average temperature affected on the DI in early barley growing stage before wintering. However any factors was not correlated with DI during wintering season that means barley growth was closely related to virus reproduction. Significant correlation between the highest temperature and DI during barley regeneration time that temperature, especially the highest, has to be considered to set appropriate time for DI investigation.