This experiment was conducted to find out the effect of day length on the production of high quality plug seedlings in onion (Allium cepa L.). Two cultivars, ‘Changnyongdaego’ and‘Wolryun’, were grown to seedlings in 200-cell plug trays under 11.5, 12.5, 13.5 hours and natural day length. These seedlings were transplanted to the pot (16 cm In diameter) and grown under 16 hours day length. Number of leaves and neck diameter showed better growth in the longer than shorter day length treatments, but plant height old sheath length were retarded in the longer day length treatments. Growth such as no. of leaves, neck diameter, plant height and sheath length increased with the passage of day, but plant height and neck diameter decreased by treatment over 20 days with 13.5 hours day length. Bulbing and bulb size of onion after transplanting were enhanced in the seedlings cultured under longer day lengths. From the above results, treatment of long day length during seedling culture in plug tray can control the overgrowth and produce high quality plug seedlings