Biodiesel as methyl esters derived from vegetable oils has considerable advantages in terms of environmental protetion. In the present work, methyl esters were produced from soybean oil by lipase-catalyzed transesterification. To reduce inactivation of commercial immobilized lipases emulsified two-step process was developed using the stepwise addition of methanol with 4:1 molar ratio at 4h intervals. Also with immobilized lipase from Candida antarctica(Novozym 435) high conversion of 98.5 percent was possible at $45^{circ}C$ of reaction temperature with 4:1 of methanol-to-oil molar ratio and 1%(v/v) methyl glucoside oleic polyester as an emulsifier in the presence of cosolvent.