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Isolation and Characterization of a Chitinolytic Enzyme Producing Marine Bacterium, Aeromonas sp. J-5003
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  • Isolation and Characterization of a Chitinolytic Enzyme Producing Marine Bacterium, Aeromonas sp. J-5003
저자명
Choi. Yong Un,Kang. Ji Hee,Lee. Myung Suk,Lee. Won Jae
간행물명
Journal of fisheries science and technology
권/호정보
2003년|6권 1호|pp.1-6 (6 pages)
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한국수산과학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A chitinolytic enzyme-producing bacterium was isolated from sea water on the coast of Busan. The bacterium was identified as Aeromonas sp. based on its morphological, cultural and biochemical characteristics and designated Aeromonas sp. J-5003. The strain produced two chitinoloytic enzymes: chitinase and chitobiase. The optimum culture conditions of the strain for production of chitinoloytic enzymes were investigated. For the production of chitinase, the major components of medium were colloidal chitin $0.5\%$, glucose $0.2\%$, yeast extract $0.25\%$ and peptone $0.25\%$ while for the production of chitobiase, they were colloidal chitin $0.5\%$, galactose and tryptone $0.2\%$. The optimum cultural temperature and initial pH for the production of chitinase and chitobiase were $30^{circ}C$ and pH 7.0, respectively.