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Amperometric Detection of Hydroquinone and Homogentisic Acid with Laccase Immobilized Platinum Electrode
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  • Amperometric Detection of Hydroquinone and Homogentisic Acid with Laccase Immobilized Platinum Electrode
  • Amperometric Detection of Hydroquinone and Homogentisic Acid with Laccase Immobilized Platinum Electrode
저자명
Quan. De,Shin. Woon-Sup
간행물명
Bulletin of the Korean Chemical Society
권/호정보
2004년|25권 6호|pp.833-837 (5 pages)
발행정보
대한화학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

DeniLite$^{TM}$ laccase immobilized platinum electrode was used for amperometric detection of hydroquinone (HQ) and homogentisic acid (HGA) by means of substrate recycling. In case of HQ, the obtained sensitivity is 280 nA/ ${mu}$M with linear range of 0.2-35 ${mu}$M ($r^2$ = 0.998) and detection limit (S/N = 3) of 50 nM. This high sensitivity can be attributed to chemical amplification due to the cycling of the substrate caused by enzymatic oxidation and following electrochemical regeneration. In case of HGA, the obtained sensitivity is 53 nA/ ${mu}$M with linear range of 1-50 $[mu}M;(r^2$ = 0.999) and detection limit of 0.3 ${mu}$M. The response times ($t_{90%}$) are about 2 seconds for the two substrates and the long-term stability is 60 days for HQ and around 40-50 days for HGA with retaining 80% of initial activities. The very fast response and the durable long-term stability are the principal advantages of this sensor. pH studies show that optimal pH of the sensor for HQ is 6.0 and that for HGA is 4.5-5.0. This shift of optimal pH towards acidic range for HGA can be attributed to the balance between enzyme activity and accessibility of the substrate to the active site of the enzyme.