Alkaloid producing species of plants have long been a major component of the medicinal social and magico-religious aspects of human culture. A diverse array of biological activities has been attributed to different alkaloids including numerous members of benzylisoquinoline family of alkaloids. For biotechnological approaches of alkaloid biosynthesis in poppy family, plant regeneration protocol through somatic embryogenesis or shoot organogenesis is a first step. This paper describes the methods and applications of plant regeneration of poppy family.