This study was carried out to determine the optimum culture conditions for Phellinus spp. known as white rot fungi showing anti-cancer activity. The optimum solid medium for mycelial growth at $25^{circ}C$ was potato dextrose agar medium and optimum pH range was $6.0{sim}8.0$, while all species showed reduced or no growth at pH 4.0. Most species showed good growth at $25{sim}30^{circ}C$. Out of 10 species of Phellinus examined, P. biscuspidatus was the best growing fungus in the range of pH $6.0{sim}7.0$ based on mycelial density. Three species such as P. biscuspidatus, P. johnsonianus and P. lloydii could be grouped in mesophile fungi, showing $30{sim}35^{circ}C$ optimum temperature.