Recently, as steel structures become higher and more long-spanned, application of high-strength steels is increasing gradually. However, criteria and example for design of high-strength steel are not built up. exiting criteria for structural steels is not proper for economical design of high-strength steel. Moreover, exiting criteria will be decrease the fatigue performance of steel bridge using high-strength steel. Therefore, criterion for application of high-strength steel must be established. In this paper, the behavior of plate girder using high-strength vertical stiffeners was clarified by carrying out layer elastic-plastic finite element analysis using finite deformation theory. In order to optimize the design and construction of plate girder using high-strength vertical stiffener, criterion for application of high-strength vertical stiffener is proposed.