In order to measure the retina thickness, there are to need the 3-D retina photographing and the high speed signal processing system. In this paper, we introduced the retina photographing system and the 16-channel high speed image data acquisition system. There are able to measure the retina thickness with sensing image the returned laser signal to APD sensor after there were projected the HeNe laser on retina. We developed the laser projection system to sense a reflected image of the retina using APD sensor, the 16-channel high speed A/D converter and PCI master interface card for image transmission into computer.