To obtain a good tracking performance in an optical disk drive servo system, it is essential to attenuate periodic disturbances caused by eccentric rotation of the disk. As an effective control scheme for enhancing disturbance attenuation performance, disturbance observers (DOBs) have been successfully applied to the track-following servo system of optical disk drives. In disk drive systems, the improvement of data transfer rate has been achieved mainly by the increase of disk rotational speed, which leads to the increase of the disturbance frequency. Conventional DOBs are no longer effective in disk drive systems with a high-speed rotation mechanism because the performance of conventional DOBs is severely degraded as the disk rotational frequency increases. This paper proposes a new DOB structure for effective rejection of the disturbance in optical disk drives with a very high rotation speed. Asymptotic disturbance rejection is achieved by adopting a band-pass filter in the DOB structure, which is tuned based on the information on the disturbance frequency. In addition, performance sensitivity of the proposed DOB to changes in disk rotational frequency is analyzed. The effectiveness of the proposed DOB is verified through simulations and experiments using a DVD-ROM drive.