The etiological agent is Bacillus anthracis, a gram-positive rod-shaped bacterium able to form spores. In order to elucidate the mechanism of infecttion on human macrophage cells, we performed two-dimensional electrophoresis and MALDI-TOF analysis using the infected human macrophage cells with the spores of B. anthracis Sterne of inactivated B. anthracis Sterne. We identified 9 proteins which related to the infection of Bacillus anthracis spores on human macrophage cells at the early stage events. Maybe nine proteins will be bio-markers and vaccine candidates to the Bacillus anthracis spore infection.