Many studies have shown that Letinus edodes has some effect on reducing blood pressure and lipid levels of animals with high pressure and hyperlipidemia. The cost of Letinus edodes varies depending on when it is harvested, and yet there has been no data collected on the bio-functional effects of Letinus edodes harvested at different times. This study was conducted to investigate the supplementary effects of Letinus edodes harvested at different times on blood pressure and blood lipid levels of hypertensive rats. Three kinds of experimental diets - control (CO), early harvested Lentinus deodes (EL), and late harvested Lentinus edodes (LL) - were supplied to spontaneously hypertensive rats (SHR) for eight weeks. The diet intake, body weight, organ weight, and serum lipid levels were subsequently measured. No significant difference was observed in diet intake and in the respective weights of the body, liver, and epididymal fat pad among experimental groups. The levels of systolic and disastolic blood pressure were significantly lower in the LE and LL groups than those in the CO group; and the concentration of serum total cholesterol was also lower in the LE and LL groups than in the CO group. These results confirm that Lentinus edodes reduces blood pressure levels and serum total cholesterol concentration in spontaneously hypertensive rats. But the effects of Lentinus edodes did not differ significantly based on whether the Lentinus edodes was harvested early or late.