Kenaf (Hibiscus cannabinus L.), an annual plant of the family Malvaceae, is considered to be the most promising for alternative plants with potential use as a non-wood fiber source. The objectives of this study were to select the optimum seeding date and adaptable cultivar among newly introduced kenaf cultivars (Everglades-41, Tainung-2 and Chingpi-3) in Jeju island, and to clarify the photosynthetic characteristics of those cultivars. Among the three cultivars, the fresh shoot weight per unit area of Chingpi-3 at harvest season showed highest through all seeding dates, that of while Everglades-41 was the lowest. The difference of shoot yield at harvest mainly due to seedling stand rate and plant number per unit area among the cultivars. The Chingpi-3 showed the highest shoot fresh weight in the seeding date of May 11 and decreased trend in the late seeding dates. Net photosynthesis rate was higher on Everglades-41 with entire type leaf than other two cultivars with palmate type leaf. The activity of ribulose-1,5-bisphosphate carboxylase/foxygenase (rubisco) estimated from the A-Ci curve showed highest in Chingpi-3 among three cultivars. These results suggest that Chingpi-3 might be adaptable cultivar with seeding date of around May 10 for kenaf production in Jeiu island.