Cigarette smoke causes atypical structure of pulmonary (cell structural) and oxidative damage. Therefore, we carried out to determine if exposure to cigarette smoke alters pulmonary structure and anti-oxidant related enzyme in a ICR mice model, when natural product extracts using by manual sprayer. The mice were divided into five groups, control group, sham-treated group (Sham), natural product extracts-treated group (NPE), natural product extracts-treated with smoke-exposed group (NPE-SM) and smoke-exposed (SM) group. All groups are similar to control group in weight, but SM group is lower than the other groups. Microscopic image of the pulmonary structure in SM group showed deleterious alterations in the morphology, but the other groups are maintained in original structure. In anti-oxidant related enzyme, SOD (superoxide dismutase) and catalase, SM group represents the lowest enzyme activity among all groups. These results indicate that the natural product extracts is an efficient tissue protective agent against smoke-induced lung injury.