Mechanical testing methods to determine the material constants for large deformation nonlinear finite element analysis were demonstrated for natural rubber. Uniaxial tension, uniaxial compression, equi-biaxial tension and pure shear tests of rubber specimens are performed to achieve the stress-strain curves. The stress-strain curves are obtained after between 5 and 10 cycles to consider the Mullins effect. Mooney and Ogden strain-energy density functions, which are typical form of the hyperelastic material, are determined and compared with each other. The material constants using only uniaxial tension data are about 20% higher than those obtained by any other test data set. The experimental equations of shear elastic modulus on the hardness and maximum strain are presented using multiple regression method. Large deformation finite element analysis of automotive transmission mount using different material constants is performed and the load-displacement curves are compared with experiments. The selection of material constant in large deformation finite element analysis depend on the strain level of component in service.