A 9 year-old male Maltese with dysuria, pollakiuria, and stranguria was brought to the Veterinary Teaching Hospital, Chonnam National University. A right lateral abdominal radiograph was shown that 3 uroliths existed at the caudal aspect of the os penis and 5 uroliths existed in the passage of os penis. Uroliths were extracted by voiding urohydropropulsion and cystotomy in Maltese dog. The complication of cystotomy was hematuria, which might persist up to 2 days postoperatively but dysuria was not observed. So we applied voiding urohydropropulsion and cystotomy, and got a good prognosis. Gross examination of extracted uroliths were separated one from another but it was identified the same componet of calcium oxalate by stone quantitative analysis.