Cotton fabrics treated with chitosan showed great improvement in fabric properties. When the add-on ratio over 0.647%, finished cotton fabrics had superior antibacterial activity. However, cotton fabrics treated by chitosan solution of high concentration exhibited increased bending rigidity and change in several physical and mechanical properties, but finished cotton fabrics had a hard touch. So, silicon softener was added to improve touch. The outstanding properties of cotton fabric treated with chitosan silicon solution had antibacterial activity, unique soft hand and durable press property. This experiment was proceed through 1 step process with chitosan and silicon softener. 1 step process takes the advantage of this experiment. Finished cotton fabrics were investigated; antibacterial activity test, moisture regain test, KES-Fb system(Kawabata Evaluation System, Kato Tech. Co. Ltd., Japan) test, scanning electron microscope(SEM). It was confirmed that the hand values of finished cotton fabrics were excellent in the CS-1