This study is concerned with the thermal analysis during the cool-down period of 135,000㎥ class GT-96 membrane type LNG carrier under IMO and USCG design condition. During the cool-down period, the spraying rate for the NG cooling decreases as the temperature of NG falls down from -4$0^{circ}C$ to -l3$0^{circ}C$, and the spraying rate for the cooling of the insulation wall increases as the temperature gradient of the insulation wall is large. It was confirmed that there existed the largest temperature decrease at the first barrier and the first insulation, which are among the insulation wall, especially in the top side of the insulation wall under IMO and USCG design condition. Also, as the NG temperature distribution is fixed, the outer temperature condition under the design condition has influence on the temperature variation at the insulation. By the 3-D numerical calculation about the cargo tank and the cofferdam during the cool-down period, the temperature variation in hulls and insulations is precisely predicted under IMO and USCG design condition. From the comparison between two conditions; IMO design condition shows more severe temperature gradient than USCG design condition, therefore, it provides the conservative estimation of the BOG.