The influence of eccentric(=staggeredness) ratio between stationary upstream circular cylinders on heat transfer characteristics of a heated downstream circular cylinder installed in a channel was investigated experimentally. In order to enhance the heat transfer rate of the heated downstream cylinder surface, we have changed the configuration of upstream cylinder. As a result, we were able to obtain local time-averaged convective heat transfer enhancement of the heated cylinder by the relative replacement of upstream cylinder. This is basically attributed to the mean flow structure change, such as flow separation, vortex shedding, and recirculation of the upstream cylinder including the reattachment and new thermal boundary developed at the downstream cylinder which are the results of the increase of the staggeredness ratio.