In this paper, the characteristics of compensation for interferenced mid-channel signal by neighbor channels through four-wave mixing (FWM) process dominantly is investigated as a function of channel input power, fiber dispersion coefficient and transmission length in WDM system with equally spaced channels. The compensation method used in this research is mid-span spectral inversion(MSSI). The highly nonlinear dispersion shifted fiber (HNL-DSF) is used as a nonlinear medium of optical phase conjugator (OPC) in order to compensate wideband WDM signals. First, we confirmed that the effect of FWM on channel interference is gradually reduced as fiber dispersion coefficient becomes gradually smaller, independent of signal format. And, we confirmed that RZ is better than NRZ as a modulation format for transmitting high power channel with allowable reception quality. But realization of flexible WDM systems regardless of channel number variation is possible by using NRZ rather than RZ format.