Nodal explants of Spilanthes acmella produced normal multiple shoots when cultured vertically on Murashige and Skoog medium (1962) supplemented with 0.5 mg L$^{-1}$ BA. The number of shoots formed from each explant was doubled after first 5-week subculture. The nodal explants placed vertically in Erlenmeyer flask (250, 500, or 1000 mL) produced more multiple shoots than those cultured in 350 mL jam bottles and 500 mL tex-Z flask. Temperature above 28$^{circ}C$ caused abnormalities of in vitro plantlets. All in vitro plantlets survived after acclimatized and transferred to the outside environment The survived plantlets did not show any morphological abnormalities in the field condition.