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High Frequency In Vitro Regeneration of Kigelia pinnata L. Via Organogenesis
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  • High Frequency In Vitro Regeneration of Kigelia pinnata L. Via Organogenesis
  • High Frequency In Vitro Regeneration of Kigelia pinnata L. Via Organogenesis
저자명
Puthur. Jos T.,Thomas. T.Dennis
간행물명
Journal of plant biology
권/호정보
2004년|47권 1호|pp.48-51 (4 pages)
발행정보
한국식물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

An effective and reproducible protocol for the micropropagation of Kigelia pinnata L. through high frequency callus regeneration is described. Seeds were surface sterilized before culturing on Gamborgs basal medium (85 medium). After two weeks the cotyledonary node along with a portion of the hypocotyl were carefully excised from well-developed embryos and subcultured on 85 medium supplemented with different concentrations of 2,4-D and BAP. The cultured cotyledonary node explants showed callus formation at the base of the lower cut end of the hypocotyl. This callus showed shoot initiation after two weeks of subculture on the regeneration medium supplemented with various concentrations of BAP alone or in combination with NAA. The highest number of shoot regeneration occurred on medium containing 5 $mu$M BAP and 0.1 $mu$M NAA. The optimum rooting of the regenerated shoots was observed on $frac{1}{2}$B5 medium supplemented with 4 $mu$M IBA. Micropropagated plants were successfully established in soil in field condition with a survival frequency of 100%.