Polysaccharides and saponins were isolated from the root of Panax ginseng C.A. Meyer (Family Araliaceae), treated at various temperatures, and their inhibitory effects on rotavirus were investigated. As the temperature of processing increased, the molecular weight of the polysaccharides decreased, but the yields of water extracted increased. These polysaccharides inhibited rotavirus infection in MA104 cells, but there were no significant differences in rotavirus infection-inhibitory potency. However, ginseng saponins did not exhibit rotavirus infection-inhibitory activity.