The sealing performance of O-rings is affected by working conditions such as applied pressure, operation temperature, pre-compressed ratio and material properties. In this paper, a pressurized and compressed elastomeric bi-polymer O-ring in which is inserted into a rectangular groove is analyzed by non-linear MARC finite element program based on the Taguchi experimental method. O-rings with 9 different profile models are analyzed for design parameters that are related to the diameter ratio between outer diameter and inner one of bi-polymer O-ring, compressive ratio, groove angle and groove depth. The calculated FEM results showed that the affection ratio of design parameter dlD, which may control sealing pressure of O-rings, is the most influential parameter among the groove angle, groove depth and compression ratio.