The purpose of this study was to the Pre-Post maximal exercise for analyze the Physiological Variable(Lactate, LDH, Glucose) in the runners. The subjects were 12 volunteers 6 sprinters and 6 distance runners who agreed to intention of this study. Subjects performed until possible all-out trials. Blood samples were taken from a median cubital vein Pre-Post exercise and every rest 5min during the all-out trial. The results obtained were summarized as follow ; 1) Lactate of Pre-Post exercise in treadmill test showed no significant difference between sprinters and distance runners, but showed significant in sprinters and distance respectively 2) LDH of Pre-Post exercise in treadmill test showed no significant difference between sprinters and distance runners, and that showed no significant in sprinters and distance respectively 3) Glucose of Pre-Post exercise in treadmill test showed no significant difference between sprinters and distance runners, and showed no significant in distance, but showed significant in sprinters therefor, since the runners has an intermittent characteristic which includes a frequent momentary change, it needs the fitness training that being consisted of various training, in the training method, it needs the training process which is from the whole fitness to specific fitness to improve general physical ability.