Objective : Angelica species are some of the most medicinally important materials in Oriental medicine. This study was performed to determine if Angelica species could be identified by genetic analysis and to verify Pyrosequencing analyses, which were used to assess genetic variation. Methods : The DNAs of Angelica acutiloba, Angelica gigas and Angelica sinensis were extracted. We have investigated the typing of single-base variations of Angelica species in DNA by using Pyrosequencing. Results : Angelica gigas showed a different pattern compared with Angelica acutiloba and Angelica sinensis. The peak of Angelica gigas was very weak in the second C nucleotide base compared with that of the others. The peak of Angelica acutiloba was present in the fourth C nucleotide base compared with that of the others. From these results we verified that our Angelica species-specific sequencing primer was well designed. Conclusion : Pyrosequencing analysis might be able to provide the identification of the Angelica species.