The aim of this study was to investigate the effect of Adenophorae Radix (AR) on melanogenesis of B16F10 mouse melanoma cells. The cells were treated for 5 days with AR at several concentrations. Treatment with AR suppressed melanin contents as a dose dependent manner without cytotoxicity and morphological change. And the extract of AR also inhibited tyrosinase activity, a key enzyme forming melanin, in a dose-dependent manner, Treatment of the cells with Adenophorae Radix also suppressed the increase of α-MSH (10 nM)-induced melanin contents and tyrosinase activity. These results suggest that AR inhibits melanogenesis and abrogates α-MSH-induced melanogenesis in B16 melanoma cells.