The aim of this study is to investigate the effect of Naegwan-Acupuncture stimulation on the relationship of change in electrocardiopgraphy(EGG). For this purpose, 11 normal volunteers were acupunctured at Naegwan acupoint using the method of Young-Su-Bo-Sa. Then, we measured and observed the change of augmented unipolar limb leads aVR, aVL and aVF in EGG. In aVR, Naegwan acupuncture treated groups were increased the activity of PR interval and PR segment compared to the control group. In aVL, Naegwan acupuncture treated groups were increased the activity of R wave amplitude, PR interval and PR segment. In aVF, Naegwan acupuncture treated groups were increased the activity of S wave amplitude and PR interval. These results suggested that Naegwan acupuncture stimulation play an important role to the activities of EGG.