Remotely Operated Vehicle of 6000-meters is a new conceptual equipment made to replace the manned systems for investigating the deep-sea environment, and all of the ROV systems in operational condition strongly depend on the connecting cables. In this point of view dynamics of the ROV cable system is very important for operational and safety aspects as a cable generally encounters great tension. Researches have been executed on this problem, and most of papers have been mainly focused on the operational condition of ROV system in deep sea. This paper presents the dynamic cable response analysis during ROV launching condition rather than the operational one in order to provide the design guide of a ROV cable system in this circumstance, considering the coupling effects between cable and wave-induced ship motion. To obtain the variations of cable tensions during a ROV launching, a pre-stressed harmonic response analysis was carried out. Wave-induced tensions of the cable during ROV launching were obtained in real sea states using FE modeling, and the basic design guide of a ROV cable system was obtained.