End-milling have been widely used in aircraft, automobile part and moulding industry. However, various working factors such as spindle speed, feed rate and depth of cut in end-milling have an effect on spindle vibration. There it is demanded the quantitative analysis of spindle vibration in order to get the optimum surface roughness. This study was carried out to analyze an influence of working factors on spindle vibration by design of Experiment. The results are shown that mathematical model of regression equation for an influence of working factors on vibration acceleration of spindle in end-milling by regression analysis is presented.