Non-woven fabrics of electrospun fibers show larger specific surface area and much smaller pore size than conventional ones, which make them excellent candidates for filtration media and membrane applications. Electrospinning combines the charging of polymer and the spinning of the fibers as an one-step process. We made the filter media from poly(acrylonitrile-co-methylacrylate) via electrospinning and evaluated the filtration characteristics. The filtration efficiency and the pressure loss increased with higher level of applied voltage, collector speed, and calendering pressure. This resulted from the decrease of pore diameter. Especially, the increase in basis weight of the web can improve the filtration efficiency without rapid pressure loss.