This study was conducted to examine the growth and physiological characteristics of Asplenium scolopendrium native fern as affected by irrigation times and soil media as an environment modeled on habitate where was sunken-condition. 1. Light intensity was lower in sunken than in non-sunken, but air humidity was higher in sunken about $2040\%$. Soil moisture content was higher with the leaf mold in sunken irrigating 2 times/week. The results of chemical analysis of medium showed that EC, pH, organic matter content, total nitrogen, CEC, Exch-Ca, Exch-Mg and Exch-K were higher with leaf mold than sud: leafmold and field soil: sud: leaf mold. 2. In the case of irrigation 2 times/week Asplenium scolopendrium grew well sunken more than non-sunken. As non-sunken condition similar with, 7 times/week irrigation, plant height, frond width, frond length and stipe length increased. In case of soil media, growth of Asplenium scolopendrium was better with leaf mold than that of sand: leafmold or field soil: sand: leaf mold. 3. In the case of irrigation 2 times/week photosynthetic rate, $CO_2$ absorption rate and water efficiency were higher with non-sunken than that of sunken, expect of stomatal conduction, $CO_2$ use efficiency. The physiological characteristics of Asplenium scolopendrium were highest in non-sunken irrigating 7 times/week In case of soil media, physiological activity was higher with leaf mold than sand: leafmold or field soil: sand: leaf mold.