The measurement of RTT (Round Trip Time) can be used for the analysis of Internet congestion. However, simple measuring of RTT which measures only hun around time of a packet can not infer a packet forward/backward delay variation. In this thesis, we present a new algorithm which can be used for the estimation of forward/backward delay variation of packets. These delay variations are implication of network congestion state. In this algorithm, the reference forward/backward delay can be determined based on the minimum RTT value. The delay variation of each packet can be calculated by comparing reference delay with the packet delay. We verified our proposed algorithm by NS-2 simulation and delay measuring in a real network.