In order to investigate the effect of premixed gasoline, diesel fuel, and n-heptane charges on the combustion and exhaust emission characteristics in a direct injection (DI) diesel engine, the experimental studies are performed. The premixed fuels are injected into the premixing chamber that installed upstream of the intake port in order to minimize the inhomogeneity effect of premixed charge. The injection nozzle for directly injected fuel is equipped in the center of the combustion chamber. The air temperature control system is equipped in the intake manifold to examine the effect of air temperature. The experimental results of this study show premixing fuel is effective method to reduce the NOx and soot emissions of diesel engine. NOx emissions are linearly decreased with increasing premixed ratio for the three kinds of premixed fuels. The heating of intake air $(80^{circ}C)$ reduced the deterioration of BSFC in high premixed ratio, because it promotes evaporation of premixed diesel droplet in the premixing chamber.