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Molecular Typing of Listeria monocytogenes Isolated from Different Sources by Pulsed-Field Gel Electrophoresis
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  • Molecular Typing of Listeria monocytogenes Isolated from Different Sources by Pulsed-Field Gel Electrophoresis
  • Molecular Typing of Listeria monocytogenes Isolated from Different Sources by Pulsed-Field Gel Electrophoresis
저자명
Kim. Hwan Deuk,Lee. Jae Youl,Suh. Dong Kyun
간행물명
Journal of experimental & biomedical sciences
권/호정보
2005년|11권 2호|pp.121-128 (8 pages)
발행정보
대한의생명과학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A total of 30L monocytogenes strains from different sources including 13 strains isolated from the foreign imported meat were genotyped in order to establish their genetic relatedness and to compare them with the foreign isolates. PFGE analysis of genomic DNA showed the $11~16$ fragments ranging in size from 38 to 504 kb. Eleven different PFGE types $(1~11)$ were identified in the dendrogram at $75\%$ similarity, and the two major PFGE types, type 1 and 2, contained $94\%$ of domestic isolates (16/17). All isolates from domestic beef and pork carcass were grouped in each different type, however, isolates from chicken were clustered together with those from pork and beef. We also found all foreign strains were unrelated with each other, regardless of geographic criteria and that they could be differentiated from those from the domestic isolates by PFGE pattern. The PFGE pattern of one isolate from chicken wing, which the chicken meat was found to be imported from foreign country, was closely related to that of isolate from the Thailand.