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Structural Analysis and Transcriptional Regulation of the Chloroplast psbC Gene from Panax ginseng
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  • Structural Analysis and Transcriptional Regulation of the Chloroplast psbC Gene from Panax ginseng
  • Structural Analysis and Transcriptional Regulation of the Chloroplast psbC Gene from Panax ginseng
저자명
Yoo. Ki-Yeol,Tae. Gun-Sik
간행물명
Journal of photoscience: an international journal officail organ of the Korean Society of Photoscience
권/호정보
2005년|12권 3호|pp.129-133 (5 pages)
발행정보
한국광과학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

The psbC gene, encoding the intrinsic chlorophyll-binding protein of CP43, one of the PS core complex polypeptides, was cloned from the Panax ginseng chloroplast, which is composed of 1,422 nucleotides and the overall nucleotide sequence shows more than 84% identity to those of eukaryotic photosynthetic organisms. The predicted topology of CP43, based on hydropathy analysis, includes six membrane-spanning ${alpha}-helices$ resulting in three lumenal and four stromal loops. The putative translation start codon for the psbC gene is located at 48 nucleotides upstream from the stop codon of the psbD gene whose product is also a component of the PSII reaction center, implying that the promoter of the psbC gene is possibly located in the middle of the structural gene of the psbD gene. Northern blot analysis of the in vivo accumulation of the psbC transcript from the plants grown under the various growth light intensities (5%, 10%, 20%, and 100%) of daylight indicated that the steady-state level of the psbC transcript was not significantly affected by light intensity.