Based on a two-degree of freedom vehicle model, this paper investigates ride comfort for a heavy off-road vehicle mounted a nonlinear hydropneumatic spring, which is influenced by nonlinear stiffness and damping characteristics of the hydropneumatic spring. Especially, the damping force is derived by applying H. Blasius formula in modeling process according to the real physical structure of the hydropneumatic spring, and the established model of nonlinear stiffness characteristics have been validated by experiments. Furthermore, the effects of parameter variations of the hydropneumatic spring, such as initial charge pressure and damping coefficient, on body acceleration, suspension deflection and dynamic tire deflection are also investigated.