It is very important to achieve a high reproducibility in the ultrasonic measurement of bone mineral density. In this study, we examined number of sampling waveform, control of temperature, diameter of region of interest as factors to improve reproducibility. We decided the optimal number of waveforms to be converted to frequency domain as period of 1. We have minimized the effects of variable temperature and constrained generation of micro bubble by keeping temperature within a range of $32pm0.5^{circ}C$ with a precise temperature controlling algorithm. We also found the optimal diameter of region of interest to be 13mm. In this paper, we demonstrated the improved reproducibility by controlling various factors affecting the ultrasonic measurement of bone mineral density.