The integrity of steam generator tubes in nuclear power plant should be maintained sufficiently during operation. For sake of this, complicated assessment procedures are required such as fracture mechanics analysis, etc. The integrity assessment of tubes has been performed by using conventional deterministic approaches while there are many uncertainties to carry out a rational evaluation. In this respect, probabilistic integrity assessment is considered as an alternative method for integrity assessment. The objectives of this study are to develop an integrity assessment system based on probabilistic fracture mechanics and to predict the failure probability of steam generator tubes containing an axial through-wall crack. The developed integrity assessment system consists of three evaluation modules, which apply first order reliability method, second order reliability method and Monte Carlo simulation method, respectively. The system has been applied to predict failure probability of steam generator tubes and the estimation results showed a promising applicability of the probabilistic integrity assessment system.