According to NERC definition, Available Transfer Capability (ATC) is a measure of the transfer capability remaining in the physical transmission network for the future commercial activity. To calculate Available Transfer Capability, accurate and defensible Total Transfer Capability, Capacity Benefit Margin and Transmission Reliability Margin should be calculated in advance. This paper proposes a method to quantify time varying Available Transfer Capability based on probabilistic approach. The uncertainties of power system and market are considered as complex random variables. Total Transfer Capability is determined by optimization technique such as SQP(Sequential Quadratic Programming). Transmission Reliability Margin with the desired probabilistic margin is calculated based on Probabilistic Load Flow analysis, and Capacity Benefit Margin is evaluated using LOLE of the system. Suggested Available Transfer Capability quantification method is verified using IEEE RTS with 72 bus. The proposed method shows efficiency and flexibility for the quantification of Available Transfer Capability