Background and Objectives : Voice perturbation measures, such as jitter and shimmer has been importantly used for diagnosis and treatment efficacy of laryngeal dysfunction. This study was conducted to investigate validity of newly developed multi-channel voice analyzer program by comparing with MDVP, PRAAT, TF32. In addition, we compared the voice perturbation measures with different voice analyzer program by type of signals. Materials and Methods : Nineteen mild-severe dysphonic patients participated in our study. Fundamental frequency, jitter and shimmer values were obtained from different voice analyzer program using the same sustained/ah/phonation. Results : Fundamental frequency and shimmer were highly correlated whereas jitter was weakly correlated between newly developed multi-channel voice analyzer program and the others though different pitch computation algorithm except MDVP, In addition, Type 2 and 3 signals were weakly correlated than Type 1. Conclusion : In the clinical setting, clinician may have sufficient information of voice analyzer and control conditions properly for severity of pathologic voice before voice perturbation measure to obtain reliable results.