A new phenolic glycoside, ophiopojaponin D (1), together with two known compounds, was isolated from the tubers of a famous traditional Chinese herb-Ophiopogon japonicus Ker-Gawl. The spectroscopic and chemical data revealed their structures to be 3-tetradecyloxy-4­hydroxy-allylbenzene-4-O-$alpha$-L-rhamnopyranosyl (1$ ightarrow$6)-$eta$-D-glucopyranoside (1), 3, 4-dihy-droxy-allylbenzene-4-O-$alpha$-L-rhamnopyranosyl (1$ ightarrow$6)-$eta$-D-glucopyranoside (2) and L-pyro­glutamic acid (3).