Background and Objectives : Vowels are characterized on the basis of formant patterns. The first formant(F1) is determined by high-low placement of the tongue, and the second formant (F2) by front-back placement of the tongue. The fundamental frequency(F0) of a soprano often exceed the normal frequency of the first formant. And the vocal intensity is boosted when F0 is high and a harmonic coincides with a formant. This is called a formant tuning. Experienced singers thus learned how to tune their formants over a resonable range by lowering the tongue to maximize their vocal intensity. So, the current study aimed to identify the formant tuning in one experienced soprano by comparing the first formants of vowel [i] in three different voice production : speech, ascending scale, and vocalize. Materials and Method : All voices recordings of vowel [i] in speech, ascending scale (from F4 note to A4 note), and vocalize(:Ridente la calam") were made with digital audio tape-corder in a sound treated room. And the captured data were analyzed by the long term average(LTA) power spectrum using the FFT algorithm of the Computerized Speech Lab(CSL, Kay elementrics, Model, 4300B). Results : Although the first formant of vowel [i] in speech was 238Hz, those of ascending scale [i] were 377Hz, 405Hz, 453Hz respectively in F4(349z), G4(392Hz), A4(440Hz) note, and 722Hz, 820Hz, 918Hz respectively in F5 (698Hz), G5(784Hz), A5(880Hz) note. In vocalize, first formants of [i] were 380Hz, 398Hz, 453Hz respectively in F4, G4, A4 note, and 720Hz, 821Hz, 890Hz respectively in F5, G5, A5 note. Conclusion : These results showed that the first formant of ascending scale and vocalize sustained higher frequency than fundamental frequency in high pitch. This finding implicates that the formant tuning of vowel [i] in ascending scale was also noted in vocalize.