The ripe unfertilized eggs of Acheilognathus signifer were elliptical shape and baggily swollen toward animal pole. The size of fertilized eggs were $2.19{pm}0.01$ mm in long axis and $1.85{pm}0.08$ mm in short axis and oil globe were not observed. The zona radiata of A. signifer had the structure of regular fibrilar net and the diameter of outer opening micropyle was about $2.5{mu}m$ and that of inner opening micropyle was about $1.5sim2.0{mu}m$. Development of fertilized eggs in A. signifer was proceeded in inclining position of animal pole and hatched in 49 hours under $20{pm}1^{circ}C$. The yolk sac was absolutely absorbed in 21 days after hatching.