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Analysis of Flavonoids in Concentrated Pomegranate Extracts by HPLC with Diode Array Detection
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  • Analysis of Flavonoids in Concentrated Pomegranate Extracts by HPLC with Diode Array Detection
  • Analysis of Flavonoids in Concentrated Pomegranate Extracts by HPLC with Diode Array Detection
저자명
Lee. Jeong-Hwan,Kim. Seung-Deok,Lee. Ja-Young,Kim. Kyung-Nam,Kim. Hyun-Su
간행물명
Food science and biotechnology
권/호정보
2005년|14권 1호|pp.171-174 (4 pages)
발행정보
한국식품과학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Three flavonoid compounds - quercetin, luteolin, and kaempferol - were analyzed from two commercially available concentrated pomegranate extracts produced in Turkey and Italy, respectively. The samples were freeze-dried and hydrolyzed by 0.4 M hydrochloric acid in 50% ethanol at $80^{circ}C$. HPLC (high-performance liquid chromatography) with DAD (diode array detection) at a wavelength of 260 nm was used for the detection of the three flavonoids. The detection limits of the three compounds were in hundreds of picograms and the signal-to-noise ratio ranged from 4 to 5: quercetin: >308 pg, s/n=4.0; luteolin: >262 pg, s/n=4.5; kaempferol: >688 pg, s/n=5.0. Quercetin, but not luteolin and kaempferol, was detected in the both pomegranate extracts. The concentrations of quercetin were $49.7;{mu}g/g$ and $27.7;{mu}g/g$ in the two pomegranate extracts made in Turkey and Italy, respectively.