We investigated the question whether protein removing activities of enzyme cleaner - protein remover for soft contact lens - are associated with the material of soft contact lens as well as action time, temperature and pH of enzyme solution. We used a subtilisin cleaner as protein remover and estimated the protein amount remained on soft contact lens after using the subtilisin cleaner under the different conditions. The remained protein in soft contact lens was greatly decreased until treatment for 60min, but no significant differences were found from 60min to 24hr. The cleaning effect of the enzymatic treatment in the range of $15{sim}30^{circ}C$ was constant. however, there was a significant decline of the protein removing effect at $10^{circ}C$ and less. The pH of the solution was also important for the efficacy of the enzymatic treatment. The activity of the enzyme cleaner was highest in pH 8.0 and significantly decreased a pH below 7. The pH dependence was found to be related to the conformational change of subtilisin. Furthermore, significant differences in the protein deposit removing efficacy of the subtilisin cleaner were found among the soft contact lens materials.