One of the most secure ways of maintaining the confidentiality and integrity of electronic information is to use electronic signatures. So, in this paper, we developed guideline on electronic signatures for EMR(electronic medical record) based on the Medical Law and the Electronic Signature Act. This guideline is intended to introduce EMR easily in the medical field and to facilitate the promotion of EMR. We developed it through consulting from the advisory committee that was made up of experts in the fields of medical record, EMR system and electronic signatures. The contents of the guideline consist of subject and time stamp of electronic signatures, validity of a certificate, management of electronic signatures and custody and management of EMR. In the future, we will develop practical cases and promote educations and publicities of them to use in the medical institutes and EMR system related industries.