We investigated the water environmental assessment by the species biotic index (SBI) of freshwater fish at the Namdaecheon in Gangneung city, Gangweon-do during June and July in 2004 and June in 2005. In fish fauna, 29 species and 10 families were collected. Dominant species was Zacco platypus (relative abundance 15.33%) and subdominant species was Rhynchocypris steindachneri (13.13%). Species biotic index (SBI) in station 1, 2, 3, 4 was 1.70, 2.85, 1.00 and 3.39 respectively and water environmental grade by SBI was all very good. Station 5 was 4.13 in SBI and good grade. Station 6 was 4.47 and fairly good. Station 7 was 7.25 and poor. And station 8 was 8.10 and very poor grade. Results of water environmental grade by SBI and water quality grade were very similar in this stream.