The antibacterial activities against Staphylococcus aureus of persimmon juice and persimmon juice dyed cotton fabrics were eveluated. We investigated the effects in pursuit of the proper treating conditions to use persimmon juice dye for comfortable and hygienic doffing. The antibacterial activities were estimated by test method KS K 0693 and the percentage reduction of bacteria at various contact conditions of test materials and bacteria. The results were as follows. no bacteriostatic activities (BS) of persimmon Juice were more than $99.98\%$ and the increase was dose-dependent according to concentration from $1\%$ to $10\%$. However, bactericidal activities (BC) were not found BS increased from $99.97\%$ to $100\%$ and BC from $79.74\%$ to $100\%$ with increasing persimmon juice concentration of cotton fabrics from $10\%$ to $100\%$. Antibacterial effects were significantly shown at $10\%$ of persimmon juices. BS increased from $75.69\%$ to $100\%$ and BC from $65.89\%$ to $100\%$ over 18 hours of contact time and continued for 50 hours. BS of $100\%$ persimmon juice dyed cotton fabrics increased from $96.56\%$ to $98.20\%$ and BC from $-136\%$ to $65\%$ at different incubation temperatures of $17pm1^{circ}C$, $27pm1^{circ}C$ and $37pm1^{circ}C$. BS ranged from $99.06\%$ to $99.99\%$ and BC from $84.31\%$ to $95.10\%$, according to the initial bacterial density from $4.08 imes10^7$ to $4.08 imes10^5$. Sixty-seven percent of subjects of answered not to smell any odor from persimmon juice dyed cotton fabrics by subjective test.