This study was conducted to investigate antioxidant enzyme activity (catalase and superoxide dismutase) and Heat Shock Protein 70 (HSP70) mRNA variation in hepatopancreas of abalone (Haliotis discus hannai) cultured under several acute water temperatures. Abalones were cultured at 10, 15, 20, 25 and $30^{circ}C$, for 0, 6, 12, 24 and 48 hours, respectively. The HSP70 mRNA expression in hepatopancreas was more increased at $30^{circ}C$ compared to those at 10. 15, 20 (control) and $25^{circ}C$. The superoxide dismutase (SOD) activity was increased in hepato-pancreas at all water temperature conditions compared to the control ($20^{circ}C$). The SOD activity at high water temperature (25 and $30^{circ}C$) tended to be increased after 12 hours, and was increased immediately after exposure to low water temperature (10 and $15^{circ}C$). and then was recovered to starting level after the increase. Also, catalase (CAT) activity in hepatopancreas was increased in all the groups except for at $10^{circ}C$ than the control ($20^{circ}C$). Survival rate of abalone was $100\%$ at 10, 15, 20 and $25^{circ}C$, but $92\%$ at $30^{circ}C$. Thus, according to our study, when abalone is appeared at $20^{circ}C$, defense mechanism against stress at low water temperature can be accelerated to be stabilized at about $5^{circ}C$. In the case of exposure of abalone to high water temperature, antioxidant enzyme and HSP70 expression were increased due to elevated physiological stimulation factor, such as temperature.